TY  - JOUR
AB  - Hydroxyproline-rich glycoproteins (HRGPs) constitute a major group of proteins of the extracellular matrix (ECM). The multicellular green algaVolvox carteriis a suitable model organism in which to study the evolutionary transition to multicellularity, including the basic principles and characteristics of an ECM. InVolvox, the ECM is dominated by a single HRGP family: the pherophorins. Our inventory amounts to 117 pherophorin-related genes inV. carteri. We focused on a pherophorin with an unexpected characteristic: pherophorin-S is a soluble, non-cross-linked ECM protein. Using transformants expressing a YFP-tagged pherophorin-S we observed the synthesis and secretion of pherophorin-S by somatic cellsin vivo, and we then traced the protein during its conspicuous migration to the ECM around prehatching juveniles and its localized concentration there. Our results provide insights into how an ECM zone surrounding the progeny is remotely affected by distantly located parental somatic cells. In view of the properties and migration of pherophorin-S, we conclude that pherophorin-S is likely to act as an ECM plasticizer to allow for dynamic ECM remodeling.
DA  - 2020
DO  - 10.1111/tpj.14901
KW  - extracellular matrix (ECM)
KW  - Volvox carteri
KW  - pherophorins
KW  - sex-inducer
KW  - protein
KW  - green algae
KW  - confocal laser scanning microscopy
KW  - multicellularity
KW  - Chlamydomonas reinhardtii
LA  - eng
IS  - 6
M2  - 2301
PY  - 2020
SN  - 0960-7412
SP  - 2301-2317
T2  - Plant journal
TI  - Targeted migration of pherophorin-S indicates extensive extracellular matrix dynamics in Volvox carteri
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-29454470
Y2  - 2024-11-22T06:16:07
ER  -