TY  - JOUR
AB  - <jats:title>ABSTRACT</jats:title>
<jats:p>The pSETT4 vector integrates into the <jats:italic>Actinoplanes</jats:italic> sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in other <jats:italic>Actinomycetales</jats:italic> by exchanging the promoter.</jats:p>
DA  - 2020
DO  - 10.1128/mra.00596-20
LA  - eng
IS  - 39
PY  - 2020
T2  - Microbiology Resource Announcements
TI  - pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-29461574
Y2  - 2024-11-22T07:59:07
ER  -