TY  - JOUR
AB  - Pickering emulsion systems have emerged as platforms for the synthesis of organic molecules in biphasic biocatalysis. Herein, the catalytic performance was evaluated for biotransformation using whole cells exemplified for the dehydration of n -octanaloxime to n -octanenitrile catalysed by an aldoxime dehydratase (OxdB) overexpressed in E. coli . This study was carried out in Pickering emulsions stabilised solely with silica particles of different hydrophobicity. We correlate, for the first time, the properties of the emulsions with the conversion of the reaction, thus gaining an insight into the impact of the particle wettability and particle concentration. When comparing two emulsions of different type with similar stability and droplet diameter, the oil-in-water (o/w) system displayed a higher conversion than the water-in-oil (w/o) system, despite the conversion in both cases being higher than that in a "classic" two-phase system. Furthermore, an increase in particle concentration prior to emulsification resulted in an increase of the interfacial area and hence a higher conversion. © 2020 Wiley-VCH GmbH.
DA  - 2021
DO  - 10.1002/anie.202013171
LA  - eng
IS  - 3
M2  - 1450
PY  - 2021
SP  - 1450-1457
T2  - Angewandte Chemie International Edition
TI  - Effect of Particle Wettability and Particle Concentration on the Enzymatic Dehydration of n-Octanaloxime in Pickering Emulsions
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-29487583
Y2  - 2024-11-21T23:13:08
ER  -