TY  - JOUR
AB  - Exposure of Chinese hamster ovary cells (CHO) to highly concentrated feed solution during fed‐batch cultivation is known to result in an unphysiological osmolality increase (>300 mOsm/kg), affecting cell physiology and morphology. Extending previous observation on osmotic adaptation, the present study investigates for the first time potential effects of hyperosmolality on CHO cells on both population and single‐cell level. We intentionally exposed CHO cells to hyperosmolality of up to 545 mOsm/kg during fed‐batch cultivation. In concordance with existing research data, hyperosmolality‐exposed CHO cells showed a nearly triplicated volume accompanied by ablation of proliferation. On the molecular level, we observed a strong hyperosmolality‐dependent increase in mitochondrial activity in CHO cells compared to control. In contrast to mitochondrial activity, hyperosmolality‐dependent proliferation arrest of CHO cells was not accompanied by DNA accumulation or caspase‐3/7‐mediated apoptosis. Notably, we demonstrate for the first time a formation of up to eight multiple, small nuclei in single hyperosmolality‐stressed CHO cells. The here presented observations reveal previously unknown hyperosmolality‐dependent morphological changes in CHO cells and support existing data on the osmotic response in mammalian cells.
DA  - 2021
DO  - 10.1002/bit.27747
LA  - eng
IS  - 6
M2  - 2348
PY  - 2021
SN  - 0006-3592
SP  - 2348-2359
T2  - Biotechnology and Bioengineering
TI  - Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology
UR  - https://nbn-resolving.org/urn:nbn:de:0070-pub-29526398
Y2  - 2024-11-24T02:26:21
ER  -