Lindner, Kirsten ; Eichelmann, Ann-Kathrin; Matuszcak, Christiane ; Hussey, Damian J. ; Haier, Jörg ; Hummel, Richard: Complex Epigenetic Regulation of Chemotherapy Resistance and Biology in Esophageal Squamous Cell Carcinoma via MicroRNAs
Inhalt
- Results
- Specific miRNA Signatures of Resistant ESCC Affect Chemotherapy Resistance
- Co-Transfection: Synergistic Effects on Resistance to Cisplatin
- Specific miRNA Signatures of Resistant ESCC Impact on Metastatic Behaviour of ESCC
- Specific miRNA Signatures of Resistant ESCC Impact on Apoptosis and Cell Cycle
- Specific miRNA Signatures of Resistant ESCC Target Various Resistance-Relevant Pathways
- Discussion
- Materials and Methods
- Cell Culture
- Selection of miRNAs and Modulation of miRNA Expression
- qRT-PCR
- Chemotherapy Treatment and Viability Assay
- Adhesion and Migration Assays
- Apoptosis and Cell Cycle Analysis
- Target Analysis: Selection of Targets, Western Blotting and Luciferase Assay
- Statistical Analysis
- References