Ferro, Kevin ; Ferro, Diana ; Corrà, Francesca; Bakiu, Rigers ; Santovito, Gianfranco; Kurtz, Joachim: Cu,Zn Superoxide Dismutase Genes in Tribolium castaneum: Evolution, Molecular Characterisation, and Gene Expression during Immune Priming
Inhalt
- Animals and Infection Protocol
- Primer Design, RNA Extraction, cDNA Synthesis
- Quantitative Real-time PCR (qRT-PCR)
- Sequence Analysis, In Silico Promoter Studies, and Molecular Phylogeny
- SOD Activity Assay
- Statistical Analysis
- Results
- Gene Architecture of T. castaneum Cu,Zn-SOD Genes
- Evolutionary Relationship and Molecular Modelling
- Cu,Zn-SOD Gene Expression after Immune Priming and Challenge
- Discussion
- Ethics Statement
- Author Contributions
- Acknowledgments
- Funding
- References