Mardin, Wolf Arif Mithat ; Haier, Jörg ; Mees, Sören Torge: Epigenetic regulation and role of metastasis suppressor genes in pancreatic ductal adenocarcinoma
Inhalt
- Background
- Methods
- Ethics statement
- Genes of interest
- Cell lines
- Animal model and biological classification of cell lines
- Nucleic acid preparation
- Bisulfite modification
- Methylation assays
- Quantitative reverse transcriptase-PCR
- Statistical analysis
- Results
- Animal model: tumor biology of the PDAC cell lines
- Metastasis suppressor gene mRNA expression
- Methylation specific PCR
- Bisulfite sequencing PCR and direct sequencing
- Correlation between MSP and BSP-direct sequencing
- Methylation patterns
- Correlation between promoter methylation and loss of mRNA expression
- Correlation between metastasis suppressor gene mRNA expression and tumor biology
- Discussion
- Conclusions
- Additional files
- Competing interests
- Authors’ contributions
- Grants
- Author details
- References