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Neunzehn, Jörg ; Lüttenberg, Beate ; Wiesmann, Hans-Peter: Investigation of biomaterials by human epithelial gingiva cells: an in vitro study
Inhalt
Material and methods
Tissue collection
Cell culture
Titanium/Zirconium dioxide
Collagen
Cell sub cultivation on materials
Richardson staining
Haematoxylin-Eosin-staining (HE)
Immunohistochemistry
Paraffin embedding
Scanning electron microscopy
Results
Tissue preparation, culture development and sub culturing
Specimen material
Scanning electron microscopic imaging of the specimens
Energy disperse x-ray analysis (EDX) of the specimen materials
Cell characterisation
Specific cell behaviour/cell attachment
Discussion
Conclusions
Competing interests
Authors’ contributions
Acknowledgements
Author details
References