Geilen, Katja ; Sawinski, Katja ; Heilmann, Mareike; Hillmer, Stefan ; Böhmer, Maik: WRKY43 regulates polyunsaturated fatty acid content and seed germination under unfavourable growth conditions
Inhalt
- Identification of negative transcriptional regulators in ABA-dependent inhibition of seed germination.
- Molecular characterization of a wrky43 transposon insertion mutant.
- WRKY43 is expressed during seed maturation.
- Disruption of WRKY43 alters seed germination on ABA, NaCl and mannitol.
- Figure 1 Screen of ORF overexpression transcription factor collections for impairment in ABA-dependent inhibition of germination.
- Figure 2 Characterization of wrky43-1 mutant (ET5604) and WRKY43 expression analysis.
- Figure 3 Disruption of WRKY43 decreases germination on ABA but increases tolerance to NaCl, cold and mannitol.
- Figure 4 Disruption of WRKY43 increases polyunsaturated fatty acid content in TAGs and phospholipids.
- Figure 5 Disruption of WRKY43 reduces transcription levels of seed storage proteins, FUS3 and ZAT10.
- Discussion
- Figure 6 WRKY43 binds directly to ZAT10 promoter but not to FUS3 promoter.
- Methods
- Plants and growth conditions.
- Plasmid constructs and plant transformation.
- Germination assay.
- Water loss assay and root growth assay.
- Imbibition-assay via seed scanning.
- Ruthenium red staining for seed coat mucilage.
- Scanning electron microscopy.
- Transmission electron microscopy.
- Quantification of total fatty acid content and fatty acid composition of dry seeds, 8 DAF seeds and leaves.
- Microarray.
- Quantitative real-time PCR.
- Protoplast Luciferase Assay.
- Acknowledgements