Wang, Nannan ; Dhumale, Pratibha ; Chiang, Joanna ; Püschel, Andreas W.: The Sema3A receptor Plexin-A1 suppresses supernumerary axons through Rap1 GTPases
Inhalt
- Hippocampal neurons from Sema3a knockout mice form multiple axons.
- Figure 1 Hippocampal neurons from Sema3a−/− embryos extend supernumerary axons in culture.
- The knockdown of Sema3A induces cell-autonomous defects.
- Plexin-A but not Plexin-B activity is required for neuronal polarity.
- Suppression of Plexin-A1 induces multiple axons.
- Plexin-A1 acts upstream of Rap1 GTPases during neuronal polarization.
- Figure 2 Knockdown of Sema3A induces the formation of supernumerary axons.
- Figure 3 A-type plexins suppress the formation of axons.
- Discussion
- Figure 4 Knockdown of Plexin-A1 induces the formation of supernumerary axons.
- Figure 5 Knockdown of Rap1B counteracts the induction of supernumerary axons by dominant-negative Plexin-A1.
- Materials and Methods
- Figure 6 Active Rap1B recues the suppression of axon formation by constitutively active Plexin-A1.
- Figure 7 Rap1B is required for the induction of supernumerary axons by the knockdown of Plexin-A1 in hippocampal neurons.
- Figure 8 Rap1B acts downstream of Sema3A in hippocampal neurons.
- Acknowledgements