Melzer, Nico; Hicking, Gordon ; Bittner, Stefan; Bobak, Nicole ; Göbel, Kerstin ; Herrmann, Alexander M. ; Wiendl, Heinz ; Wiendl, H. ; Meuth, Sven ; Meuth, Sven G.: Excitotoxic neuronal cell death during an oligodendrocyte-directed CD8+ T cell attack in the CNS gray matter
Inhalt
- Methods
- Mice
- Isolation of splenocytes and stimulation of CD8+ T cells
- Flow cytometry
- Determination of supernatant glutamate levels
- Quantitative real-time PCR
- Neuronal cell culture and glutamate excitotoxicity assays
- Cytotoxicity assay
- Preparation of acute brain slices and co-culture experiments with CD8+ T cells
- Immunofluorescence-staining and -microscopy
- Reagent preparations
- Statistical analysis
- Results
- CD8+ T cells release glutamate by a TCR-stimulus-dependent mechanism
- Antigen-dependent glutamate release from CD8+ T cells varies with repetitive stimulation
- Antigen-dependent glutamate release from CD8+ T cells causes excitotoxic neuronal cell death during an oligodendrocyte-directed CD8+ T cell attack in the CNS gray matter
- Discussion
- Conclusions
- Additional file
- Abbreviations
- Competing interests
- Authors’ contributions
- Acknowledgements
- Author details
- References