Jagmann, Nina ; Philipp, Bodo: SpoT-Mediated Regulation and Amino Acid Prototrophy Are Essential for Pyocyanin Production During Parasitic Growth of Pseudomonas aeruginosa in a Co-culture [...]
Inhalt
- Bacterial Strains, Plasmids, and Growth Experiments
- Construction of Plasmids and Mutants of P. aeruginosa
- β-Galactosidase Experiments
- Quantification of Pyocyanin
- Transposon Mutagenesis
- Oxidative Stress Experiments
- Results
- QS-Regulated Pyocyanin Production by P. aeruginosa in the Co-culture Is Not Induced by IQS-Integrated Phosphate Limitation
- QS-Regulated Pyocyanin Production in the Co-culture Is Induced by the Stringent Response
- The Stringent Response in the Co-culture Is Mediated by SpoT
- Overexpression of PqsE Does Not Restore Pyocyanin Production by Strain PAO1relAspoT in the Co-culture
- Genes Involved in Amino Acid Biosynthesis Are Essential for Pyocyanin Production in the Co-culture
- Overexpression of PqsE Does Not Restore Pyocyanin Production by Auxotrophic Strains in Co-culture
- Discussion
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References