Janßen, Helge ; Janßen, Helge Jans ; Janßen, HJ ; Steinbüchel, Alexander ; Steinbüchel, A.: Fatty acid synthesis in Escherichia coli and its applications towards the production of fatty acid based biofuels
Inhalt
- Fatty acid biosynthesis
- The E. coli type-II FAS enzymes
- AccABCD: acetyl-CoA carboxylase
- FabD: malonyl-CoA:ACP transacylase
- FabB, FabF and FabH: 3-ketoacyl-ACP synthase I, II and III
- FabG: 3-ketoacyl-ACP reductase
- FabA and FabZ: 3-hydroxyacyl-ACP dehydrase
- FabI: enoyl-ACP reductase
- ACP, ACP synthase and ACP phosphodiesterase
- Membrane synthesis
- Fatty acid degradation
- Regulation in E. coli
- Transcriptional regulation by FadR and FabR
- Promoters of the fab-operon
- Regulation by the stringent response
- Regulation according to the growth conditions
- Production of free fatty acids
- Thioesterase expression and physiological consequences
- Deletion of β-oxidation genes
- Investigation and remodeling of the whole pathway
- Altering the regulation or pathway direction
- Process optimization
- Production of FAAE
- Production of other fatty acid-derived biofuels
- Feedstocks
- Conclusions
- Abbreviations
- Competing interests
- Authors’ contributions
- Acknowledgements
- References