Berning, Philipp ; Schäfer, Christiane ; Clemens, Dagmar; Korsching, Eberhard Ulrich ; Dirksen, Uta ; Potratz, Jenny Charlotte: The CXCR4 antagonist plerixafor (AMD3100) promotes proliferation of Ewing sarcoma cell lines in vitro and activates receptor tyrosine kinase signaling
Inhalt
- Methods
- Cell lines
- Compounds and reagents
- Migration and wound healing assays
- Flow cytometry
- Western blotting
- Phospho-receptor tyrosine kinase array
- siRNA transfection
- Real-time quantitative reverse transcription PCR
- Statistics
- Results
- Plerixafor promotes proliferation of Ewing sarcoma cell lines in vitro
- Ewing sarcoma cell lines show chemotactic migration to plerixafor
- Cell lines group into CXCR4-high and -low surface expression
- Plerixafor-induced proliferation does not require substantial CXCR4 expression but is associated with AKT activation
- Plerixafor induces phosphorylation of receptor tyrosine kinases
- Plerixafor and CXCR4 signaling interact with PDGFRB
- Discussion
- Plerixafor can promote Ewing sarcoma cell proliferation
- Cellular target receptors of pro-proliferative plerixafor actions
- Transduction of pro-proliferative plerixafor signals
- Feedback survival signaling as a novel mechanism of CXCR4 and RTK interaction
- Conclusions
- http://www.ema.europa.eu/ema/index.jsp?curl=pages/medicines/human/medicines/001030/human_med_000910.jsp&mid=WC0b01ac058001d124; https://www.accessdata.fda.gov/scripts/cder/daf/index.cfm?event=overview.process&applno=022311. Accessed Sept 18, 2017.
- Additional files
- Abbreviations
- Funding
- Availability of data and materials
- Authors’ contributions
- Ethics approval and consent to participate
- Competing interests
- Publisher’s Note
- Author details
- References