Kerl, Kornelius Tobias ; Ries, David ; Unland, Rebecca ; Borchert, Christiane ; Moreno, Natalia ; Hasselblatt, Martin ; Jürgens, Heribert ; Kool, Marcel ; Görlich, Dennis ; Eveslage, Maria ; Jung, Manfred ; Meisterernst, Michael ; Frühwald, Michael: The histone deacetylase inhibitor SAHA acts in synergism with fenretinide and doxorubicin to control growth of rhabdoid tumor cells
Inhalt
- Methods
- Cell lines
- Histone deacetylase inhibitors, Cyclin D inhibitors and chemotherapy
- Cytotoxicity assay
- Analysis of combined drug effects on cytotoxicity
- Western blots
- Antibodies for western blots
- Apoptosis detection and cell cycle analysis
- cDNA microarray experiments and statistical analysis
- Primers used for real-time PCR
- Results
- HDACs are highly expressed in primary rhabdoid tumors and rhabdoid tumor cell lines
- The non-selective histone deacetylase inhibitor SAHA induces reversible G2-arrest and apoptosis in SMARCB1 negative tumors
- SAHA induces expression of RB-, MYC- and pluripotency-associated genes
- SAHA synergizes with fenretinide in inhibiting rhabdoid cell growth
- Discussion
- Conclusion
- Additional files
- Abbreviations
- Competing interests
- Authors’ contributions
- Availability of data
- Author details
- References