Bokemeyer, Arne ; Tepasse, Phil-Robin ; Tepasse, Phil R. ; Quill, Lena; Lenz, Philipp ; Rijcken, Emile Jan Marie ; Vieth, Michael; Ding, Nik; Ketelhut, Steffi ; Rieder, Florian ; Kemper, Björn ; Bettenworth, Dominik: Quantitative Phase Imaging Using Digital Holographic Microscopy Reliably Assesses Morphology and Reflects Elastic Properties of Fibrotic Intestinal Ti [...]
Inhalt
- Study population.
- Figure 1 Experimental set-up.
- Table 1 Characteristics of patients with Crohn’s disease associated intestinal strictures undergoing surgical resection of the stricture.
- Evaluation of fibrotic alterations within tissue samples by immunofluorescence.
- Correlation between the refractive index and intestinal fibrosis.
- Figure 2 Determination of the refractive index of stenotic and non-stenotic intestinal tissue of Crohn’s disease patients using digital holographic microscopy (DHM).
- Table 2 Significantly prolonged average optical path length delay Δϕ and refractive index (RI) in stenotic compared to non-stenotic intestinal tissue of Crohn’s disease patients (surgical resection specimen n = 30 number of patients = 15).
- Figure 3 Immunofluorescence studies of non-stenotic and stenotic intestinal tissue of Crohn’s disease patients.
- Discussion
- Figure 4 Significant correlation between the refractive index (RI) determined by digital holography microscopy (DHM) and the immunofluroescence-intensity of fibrosis markers.
- Figure 5 Tissue stiffness of stenotic intestinal tissue was significantly elevated compared to non-stenotic intestinal tissue of Crohn’s disease patients.
- Methods
- Study design and inclusion criteria.
- Histological evaluation.
- Quantitative phase imaging with digital holographic microscopy.
- Determination of the refractive index using digital holographic microscopy.
- Evaluation of density changes of intestinal tissue of CD patients by digital holographic microscopy.
- Immunofluorescence studies for the assessment of fibrosis in intestinal tissue.
- Assessment of the tissue stiffness of intestinal tissue.
- Statistical analysis.
- Acknowledgements