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Vordemvenne, Thomas ; Paletta, Jürgen R. J. ; Hartensuer, René ; Pap, Thomas; Raschke, Michael J. ; Ochman, Sabine: Cooperative effects in differentiation and proliferation between PDGF-BB and matrix derived synthetic peptides in human osteoblasts
Inhalt
Methods
Isolation of human osteoblast-like cells
Stimulation of osteoblast-like cells
Proliferation and cell number
Calcification
Total RNA extraction and cDNA synthesis
Quantitative RT-PCR
Statistical Analysis
Results
Proliferation
Calcification
Gene expression experiments
Alkaline phosphatase
Collagen I
Osteocalcin
Discussion
Conclusions
Acknowledgements
Author details
Authors' contributions
Competing interests
References
Pre-publication history