Pakkianathan, Britto Cathrin ; Singh, Nitin Kumar ; Krishnan, Muthukalingan ; König, Simone: A proteomic view on the developmental transfer of homologous 30 kDa lipoproteins from peripheral fat body to perivisceral fat body via hemolymph in silkworm, [...]
Inhalt
- Background
- Methods
- Insects
- Hemolymph
- Fat body
- Protein extraction
- Gel electrophoresis
- Gel staining
- Western blot using vitellogenin antibody
- Lipoprotein staining
- Ion exchange chromatography
- Gel permeation chromatography
- Field emission scanning electron microscopy (FE-SEM)
- LC-MS/MS and data analysis
- Results and discussion
- Conclusion
- Acknowledgements
- Author details
- Authors' contributions
- Competing interests
- References