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Annussek, Tobias ; Kleinheinz, Johannes ; Szuwart, Thomas ; Joos, Ulrich; Wermker, Kai: Short time administration of antirheumatic drugs - Methotrexate as a strong inhibitor of osteoblast´s proliferation in vitro
Inhalt
Materials and methods
Cell culture
Cell Counting
MTT-assay
Immunostaining
Critical concentration identification
Statistical analysis
Results
Cell morphology and immunostaining
Cell counting
MTT-assay
Detection of critical MTX-concentration
Discussion
Competing interests
Authors´ contributions
Author details
References