Sato, Shunsuke ; Andreeßen, Björn ; Steinbüchel, Alexander ; Steinbüchel, A.: Strain and process development for poly(3HB-co-3HP) fermentation by engineered Shimwellia blattae from glycerol
Inhalt
- Materials and methods
- Strain and plasmid
- Growth of cells
- Plasmid construction and transfer into E. coli and S. blattae
- Optimization of cultivation medium
- Synthesis and purification of poly(3HB-co-3HP)
- Cell harvest and extraction of poly(3HB-co-3HP)
- Determination of poly(3HB-co-3HP)
- Determination of glycerol and 1,3PD
- Results
- Synthesis of poly(3HB-co-3HP) influenced by cultivation conditions
- Optimization of the medium for cultivation
- Synthesis of poly(3HB-co-3HP) in MMB medium
- Discussion
- Competing interests
- Authors’ contributions
- Acknowledgement
- References