Schneider, György ; Dobrindt, Ulrich ; Middendorf, Barbara ; Hochhut, Bianca ; Szijártó, Valéria ; Emődy, Levente ; Hacker, Jörg: Mobilisation and remobilisation of a large archetypal pathogenicity island of uropathogenic Escherichia coli in vitro support the role of conjugation for [...]
Inhalt
- Results
- Transfer of the entire PAI II536 from UPEC strain 536 into E. coli K-12
- PAI II536 integrates site-specifically into the E. coli K-12 chromosome at the tRNA gene leuX
- Remobilisation of the transferred PAI II536 into E. coli strain 536-21
- Discussion
- Conclusions
- Methods
- Bacterial strains and growth conditions
- Oligonucleotides
- Preparation and manipulation of DNA
- Construction of a mobilisable PAI II536 construct
- Integration of the pir gene into the λ attachment site of uropathogenic E. coli strain 536
- Mobilisation of the labelled PAI II536 by the broad host range conjugative plasmid RP4
- Conjugative transfer of PAI II536
- Analysis of candidate transconjugants for PAI II536 transfer, deletion, and integration
- Phenotypic characterisation of transconjugants
- Statistical analysis
- Acknowledgements and Funding
- Author details
- Authors' contributions
- References