Tozakidis, Iasson; Tozakidis, Iasson E. P.; Tozakidis, Iasson Elias Philippos; Brossette, Tatjana; Lenz, Florian; Maas, Ruth Maria; Jose, Joachim: Proof of concept for the simplified breakdown of cellulose by combining Pseudomonas putida strains with surface displayed thermophilic endocellulase, exocellulase [...]
Inhalt
- Glucose uptake and recyclability of P. putida at different temperatures
- Construction of MATE-cellulase fusion genes
- Expression and localization of MATE-cellulase fusion proteins
- Surface display of cellulases on P. putida
- Activity of MATE-cellulases on the surface of P. putida
- Recyclability of cellulase-displaying P. putida strains
- Concerted hydrolysis of filter paper
- Discussion
- Conclusions
- Methods
- Bacterial strains and culture conditions
- Cellulase genes and construction of MATE expression vectors
- Outer membrane protein analysis
- Flow cytometer analysis
- Exocellulase and β-glucosidase activity assay
- Endocellulase activity assay
- Reusability experiments
- Filter paper assay
- Detection of reducing sugars and glucose
- Authors’ contributions
- References