Nianios, Dimitrios ; Thierbach, Sven ; Steimer, Lenz ; Steimer, Lenz Rudolf ; Lulchev, Pavel ; Klostermeier, Dagmar ; Fetzner, Susanne: Nickel quercetinase, a “promiscuous” metalloenzyme: metal incorporation and metal ligand substitution studies
Inhalt
- Results
- Metal selectivity and catalytic activity of recombinant QueD
- QueD from the wild-type strain, Streptomyces sp. FLA
- Cell-free protein synthesis (CFPS) of QueD in the presence of metal ions
- Replacement of the 3His/1Glu ligand amino acids of Ni-QueD
- Discussion
- Conclusions
- Methods
- Materials
- DNA techniques
- Cell-free protein synthesis (CFPS)
- Expression and purification of C-terminally Strep-tagged QueD proteins
- Growth of Streptomyces sp. FLA and purification of QueD protein
- Protein analysis
- Identification of organic reaction products
- Fluorescence titration
- Circular Dichroism (CD) spectroscopy
- Additional files
- Abbreviations
- Competing interests
- Authors’ contributions
- Acknowledgments
- Author details
- References