Haas, Astrid ; Nilges, Benedikt S. ; Leidel, Sebastian A.; Klempnauer, Karl-Heinz: PDCD4 controls the G1/S-phase transition in a telomerase-immortalized epithelial cell line and affects the expression level and translation of multiple [...]
Inhalt
- PDCD4 is required for the G1/S-transition in RPE cells.
- Figure 1 PDCD4 knockdown affects the cell cycle and growth properties of RPE cells.
- Defective G1/S-checkpoint control overrides the requirement for PDCD4 to undergo G1/S-transition.
- Knockdown of PDCD4 activates the G1/S-checkpoint in RPE cells by increasing the expression of p21WAF1/CIP1.
- Figure 2 The G1/S-transition is independent of PDCD4 expression in HEK293T cells.
- Figure 3 PDCD4 is linked to the G1/S checkpoint via the p53-p21WAF1/CIP1 axis.
- Figure 4 Pdcd4 knockdown induces transcriptome-wide changes of mRNA expression.
- Discussion
- Figure 5 Gene set enrichment analysis of genes affected by PDCD4 knockdown.
- Figure 6 PDCD4 silencing affects the translational landscape of RPE cells.
- Materials and Methods
- Cells and siRNA transfections.
- Cell cycle analysis.
- 3H-thymidine labeling.
- RNA-seq.
- Ribosome profiling.
- Sequencing data analysis.
- Gene set enrichment analysis (GSEA).
- Calculation of ΔG values for 5′-UTR secondary structure formation.
- Data access.
- Acknowledgements