Kuntze, Anna ; Goetsch, Ole; Fels, Benedikt ; Najder, Karolina ; Unger, Andreas ; Wilhelmi, Marianne; Sargin, Sarah; Schimmelpfennig, Sandra; Neumann, Ilka; Schwab, Albrecht ; Pethő, Zoltán: Protonation of Piezo1 Impairs Cell-Matrix Interactions of Pancreatic Stellate Cells
Inhalt
- Materials and Methods
- Animal Experiments
- Murine PSC Isolation and Culture
- Plasmids and Transfection
- Immunocytochemistry
- Western Blot
- Calcium Influx Measurement
- Determination of the Intracellular pH
- Cell Migration and Spheroid Traction Experiments
- Spheroid Histology and Confocal Reflectance Imaging
- Assessment of Cellular Viability
- Statistics
- Results
- Piezo1 Channels Are Expressed in Pancreatic Stellate Cells
- Piezo1 Channels Facilitate Pancreatic Stellate Cell Migration and Cell-ECM Interaction
- Both Intra- and Extracellular Acidification Impair Piezo1 Activity
- Piezo1 Function in PSCs Is Affected by the Cytoskeleton
- Acidic pH Disrupts PSC Spheroid Integrity
- Discussion
- Data Availability Statement
- Ethics Statement
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References