Eichelmann, Ann-Kathrin; Mayne, George C.; Chiam, Karen; Due, Steven L.; Bastian, Isabell; Butz, Frederike; Wang, Tingting; Sykes, Pamela J.; Clemons, Nicholas J.; Liu, David S.; Michael, Michael Z.; Karapetis, Christos S.; Hummel, Richard ; Watson, David I.; Hussey, Damian J.: Mutant p53 Mediates Sensitivity to Cancer Treatment Agents in Oesophageal Adenocarcinoma Associated with MicroRNA and SLC7A11 Expression
Inhalt
- Results
- Effect of p53 Knockout on Cell Survival after Ionising Irradiation
- Effect of p53 Knockout on Cell Survival after Drug Treatment
- Alterations in MiRNA Expression in JH-EsoAd1 Cells after p53 Knockout
- Pathway Analyses
- Rationale for Studying SLC7A11, a Key Regulator of the Antioxidant Response
- SLC7A11 Knockdown Restores Radiosensitivity of p53-KO Cells
- Alterations in MiRNA Expression and Predicted Pathways Resulting from SLC7A11 Knockdown
- Interaction of MiRNAs with Oestrogen Signaling
- Discussion
- Effect of p53 Knockout on Cell Survival after Ionizing Irradiation and Drug Treatment
- Alterations in MiRNA Expression in JH-EsoAd1 Cells after p53 Knockout Followed by Pathway Analysis
- SLC7A11 Knockdown Restores Radiosensitivity of p53-KO JH-EsoAd1 Cells
- Alterations in MiRNA Expression after SLC7A11 Knockdown Followed by Pathway Analysis
- Possible Roles for Oestrogen Receptor Signaling
- Limitations
- Methods
- Cell Lines and Culture Conditions
- Irradiation and Clonogenic Survival Assay
- Drug Treatment and Apoptosis Assay
- SLC7A11 Knockdown Using siRNA
- Irradiation and Clonogenic Survival Assay Following SLC7A11 Knockdown
- Statistical Analyses
- Western Blot
- RNA Extraction
- TaqMan® OpenArray® MiRNA Profiling
- House Keeping Gene (HKG) Selection
- Sample Comparisons, for Pooling of Cell Lines
- Differential Expression Analyses
- Gene Ontology and Biological Pathway Enrichment Analysis
- Conclusions
- References