Liu, Li ; Brown, Philip C.; Könneke, Martin ; Huber, Harald ; König, Simone; Berg, Ivan A.: Convergent Evolution of a Promiscuous 3-Hydroxypropionyl-CoA Dehydratase/Crotonyl-CoA Hydratase in Crenarchaeota and Thaumarchaeota
Inhalt
- RESULTS
- Crotonyl-CoA hydratase and 3-hydroxypropionyl-CoA dehydratase reactions in M. sedula cell extracts.
- Characterization of an enoyl-CoA hydratase Msed_0566.
- Quantification of enoyl-CoA hydratase homologs in autotrophically grown M. sedula.
- Msed_2001 is a promiscuous 3-hydroxypropionyl-CoA dehydratase/crotonyl-CoA hydratase.
- Characterization of 3-hydroxypropionyl-CoA dehydratase/crotonyl-CoA hydratase Nmar_1308 from N. maritimus and its phylogenetic analysis.
- Characterization of a putative crotonyl-CoA hydratase Slip_2089 from S. lipocalidus.
- DISCUSSION
- MATERIALS AND METHODS
- Materials.
- Microbial strains and growth conditions.
- CoA-esters synthesis.
- Preparation of M. sedula cell extracts.
- Gene cloning.
- Heterologous expression in E. coli.
- Purification of recombinant proteins.
- Enzyme assays.
- Database search and phylogenetic analysis.
- Mass spectrometric expression analysis.
- Other methods.
- Data availability.
- SUPPLEMENTAL MATERIAL
- ACKNOWLEDGMENTS
- REFERENCES