Kronenberg, Daniel; Michel, Philipp ; Hochstrat, Eva; Wei, Ma; Brinckmann, Jürgen; Müller, Marcus; Frank, Andre; Hansen, Uwe; Eckes, Beate; Stange, Richard: Increased Collagen Turnover Impairs Tendon Microstructure and Stability in Integrin α2β1-Deficient Mice
Inhalt
- Results
- Integrin 21-Deficient Tenocytes Produce More Collagen and Express More Matrix and Tendon-Related Proteins In Vitro
- Increased Collagen Expression by Integrin 21-Deficient Tenocyte Like Fibroblasts Is Not Transferred to the Tissue
- Collagen Fibrils Lacking Integrin 21 Have No Increased Inherent Disorder
- Integrin 21-Deficient Tendons Show a Predominance of Small Collagen Fibrils
- Biomechanical Comparison Reveals Reduced Young’s Modulus in the Absence of Integrin 21
- Integrin 21-Deficient Tendons Showed Decreased Lysyloxidase Quantities—The Cross-Linking Pattern Appeared Not to Be Affected
- Integrin 21-Deficient Tendons Show Increased Gelatinase Activity
- Integrin 21-Deficient Tendons Contain More Soluble Collagen Fragments
- Discussion
- Materials and Methods
- Animals Used
- Histology
- Polarization Microscopy
- Transmission Electron Microscopy
- Biomechanical Testing
- Tenocyte Culture
- Quantitative Real-Time PCR
- Collagen Content in Tenocyte Generated Matrix and in Tendon Tissue
- Gelatin-/Casein Zymography of Tendons
- Lysyl Oxidase Determination
- Assessment of Collagen, Non-Collagenous Protein and Collagen Cross-Links
- Quantification of Soluble Collagen Fragments in Tendons
- Statistical Analysis
- Conclusions
- References