Weiand, Matthias; Ballmaier, Paula Johanna ; Niemietz, Christoph ; Schmidt, Hartmut ; Zibert, Andree: Combined transgene immortalized urothelial cells capable of reprogramming and hepatic differentiation
Inhalt
- 2.1 Ethics statement
- 2.2 Isolation of urine-derived cells
- 2.3 Retroviral transduction
- 2.4 Flow cytometry analysis
- 2.5 Immunocytochemistry
- 2.6 Cumulative cell growth
- 2.7 Induced pluripotent stem cells
- 2.8 Differentiation of hepatocyte-like cells
- 2.9 PCR
- 2.10 Western blot
- 2.11 Statistical analyses
- 3 Results
- 3.1 Combined transgene expression results in immortalization and increased proliferation of urine-derived cells
- 3.2 Urothelial marker expression of immortalized cells
- 3.3 Onset of iPSC formation is delayed after reprogramming of transgene expressing cells
- 3.4 Hepatic differentiation of immortalized cells reveals typical marker expression and partly reverses transgene silencing
- 4 Discussion
- Declaration of competing interest
- Acknowledgement
- Appendix A Supplementary data
- References