Glaser, Felix ; Glaser, Felix Martin ; Hundehege, Petra; Bulk, Etmar Michael ; Todesca, Luca Matteo; Schimmelpfennig, Sandra; Nass, Elke; Budde, Thomas ; Meuth, Sven ; Meuth, Sven G. ; Schwab, Albrecht: KCa channel blockers increase effectiveness of the EGF receptor TK inhibitor erlotinib in non-small cell lung cancer cells (A549)
Inhalt
- Bioinformatic analysis.
- Cell culture.
- Proliferation.
- Cell migration.
- Single-cell electrophysiology.
- Western blotting.
- Statistics.
- Results
- Altered expression of KCa3.1 channels in erlotinib-resistant NSCLC cells.
- Functional KCa channels in wildtype and partially erlotinib-resistant A549-3R cells.
- Erlotinib inhibits migration and proliferation of A549-3R cells.
- Combining erlotinib with senicapoc or paxilline elicits additive effects on migration and proliferation.
- Partial erlotinib resistance can be overcome by KCa3.1 or KCa1.1 channel blockade.
- Hypoxia augments the contribution of KCa3.1 channels to migration and proliferation.
- Discussion
- References
- Acknowledgements