Begemann, Isabell; Keller, Ulrike; Nüsse, Harald; Klingauf, Jürgen; Galic, Milos: Parallel Acquisition of Plasma Membrane Ultrastructure and Cytosolic Protein Localisation in Cultured Cells via Correlated Immunogold SEM

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Material and Methods

Holder and Sample Preparation
Fixation
Immunogold Labelling
Dehydration and Coating
Imaging

Results

Detection Depth of Back-Scattered Electrons
Validation of Localization Depth
Applications

Discussion

Comparison with Existing Methods
Limitations and Further Improvements of the Method

Conclusions
References

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