TY  - JOUR
AB  - Infection with influenza A viruses (IAV) provokes activation of cellular defence mechanisms contributing to the innate immune and inflammatory response. In this process the cyclooxygenase-2 (COX-2) plays an important role in the induction of prostaglandin-dependent inflammation. While it has been reported that COX-2 is induced upon IAV infection, in the present study we observed a down-regulation at later stages of infection suggesting a tight regulation of COX-2 by IAV. Our data indicate the pattern-recognition receptor RIG-I as mediator of the initial IAV-induced COX-2 synthesis. Nonetheless, during on-going IAV replication substantial suppression of COX-2 mRNA and protein synthesis could be detected, accompanied by a decrease in mRNA half-life. Interestingly, COX-2 mRNA stability was not only imbalanced by IAV replication but also by stimulation of cells with viral RNA. Our results reveal tristetraprolin (TTP), which is known to bind COX-2 mRNA and promote its rapid degradation, as regulator of COX-2 expression in IAV infection. During IAV replication and viral RNA accumulation TTP mRNA synthesis was induced, resulting in reduced COX-2 levels. Accordingly, the down-regulation of TTP resulted in increased COX-2 protein expression after IAV infection. These findings indicate a novel IAV-regulated cellular mechanism, contributing to the repression of host defence and therefore facilitating viral replication.
AU  - Dudek, Sabine Eva
AU  - Nitzsche, Katja
AU  - Ludwig, Stephan
AU  - Ehrhardt, Christina
DA  - 2016-06-06
DO  - 10.1038/srep27275
LA  - eng
N1  - Scientific Reports 6 (2016) 27275, 1-13
N1  - Finanziert durch den Open-Access-Publikationsfonds 2015/2016 der Westfälischen Wilhelms-Universität Münster (WWU Münster).
PY  - 2016-06-06
SN  - 2045-2322
TI  - Influenza A viruses suppress cyclooxygenase-2 expression by affecting its mRNA stability
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-64269522380
Y2  - 2024-11-24T05:41:54
ER  -