TY  - JOUR
AB  - Vitamin C and vitamin E are known as important cellular antioxidants and are involved in several other non-antioxidant processes. Generally vitamin C and vitamin E are not synthesized by humans and therefore have to be applied by nutrition. The absence or deficiency of the vitamins can lead to several dysfunctions and even diseases (e.g. scurvy). The main interest in this study is that vitamin C and E are known to influence bone formation, e.g. vitamin C plays the key role in the synthesis of collagen, the major component of the extracellular bone matrix. In the present study we evaluate the effect of ascorbic acid (vitamin C) and α-tocopherol (vitamin E) on the proliferation and differentiation of primary bovine osteoblasts in vitro. Starting from standard growth medium we minimized the foetal calf serum to reduce their stimulatory effect on proliferation. An improved growth and an increased synthesis of the extracellular matrix proteins collagen type I, osteonectin and osteocalcin was observed while increasing the ascorbic acid concentration up to 200 μg/ml. Furthermore the effects of α-tocopherol on cell growth and cell differentiation were examined, whereby neither improved growth nor increased synthesis of the extracellular matrix proteins collagen type I, osteonectin and osteocalcin were detected. Further investigations are necessary to target at better supportive effect of vitamins on bone regeneration, and healing.
AU  - Urban, Kent
AU  - Höhling, Hans-Jürgen
AU  - Höhling, Hans Jürgen
AU  - Lüttenberg, Beate
AU  - Szuwart, Thomas
AU  - Plate, Ulrich
DA  - 2012-09-28
DO  - doi:10.1186/1746-160X-8-25
KW  - Bone cells
KW  - Osteoblast
KW  - Ascorbic acid
KW  - Tocopherol
KW  - Bone regeneration
LA  - eng
N1  - Finanziert durch den Open-Access-Publikationsfonds 2012/2013 der Deutschen Forschungsgemeinschaft (DFG) und der Westfälischen Wilhelms-Universität Münster (WWU Münster).
N1  - Head & Face Medicine 8 (2012) 25
PY  - 2012-09-28
TI  - An in vitro study of osteoblast vitality influenced by the vitamins C and E
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-07369597298
Y2  - 2024-11-21T23:56:38
ER  -