TY  - JOUR
AB  - Background: The generation of antibodies is impaired in newborns due to an immature immune system and reduced exposure to pathogens due to maternally derived antibodies and placental functions. During nursing, the immune system of newborns is challenged with multiple milk-derived proteins. Amongst them, caseins are the main constituent. In particular, human aS1-casein (CSN1S1) was recently shown to possess immunomodulatory properties. We were thus interested to determine if auto-antibodies to CSN1S1 are induced by breast-feeding and may be sustained into adulthood. Methods: 62 sera of healthy adult individuals who were (n = 37) or were not (n = 25) breast-fed against human CSN1S1 were investigated by a new SD (surface display)-ELISA. For cross-checking, these sera were tested for anti Epstein-Barr virus (EBV) antibodies by a commercial ELISA. Results: IgG-antibodies were predominantly detected in individuals who had been nursed. At a cut-off value of 0.4, the SDELISA identified individuals with a history of having been breast-fed with a sensitivity of 80% and a specificity of 92%. Under these conditions, 35 out of 37 sera from healthy donors, who where breast-fed, reacted positively but only 5 sera of the 25 donors who were not breast-fed. The duration of breast-feeding was of no consequence to the antibody reaction as some healthy donors were only short term breast-fed (5 days minimum until 6 weeks maximum), but exhibited significant serum reaction against human CSN1S1 nonetheless. Conclusion: We postulate that human CSN1S1 is an autoantigen. The antigenicity is orally determined, caused by breastfeeding, and sustained into adulthood.
AU  - Petermann, Klaudia
AU  - Vordenbäumen, Stefan
AU  - Maas, Ruth Maria
AU  - Braukmann, Achim
AU  - Bleck, Ellen
AU  - Sänger, Thorsten
AU  - Schneider, Matthias
AU  - Jose, Joachim
DA  - 2012-04-04
DO  - doi:10.1371/journal.pone.0032716
LA  - eng
N1  - Finanziert durch den Open-Access-Publikationsfonds 2011/2012 der Deutschen Forschungsgemeinschaft (DFG) und der Westfälischen Wilhelms-Universität Münster (WWU Münster).
N1  - PLoS ONE 7 (2012) 4, e32716
PY  - 2012-04-04
TI  - Autoantibodies to aS1-Casein Are Induced by Breast-Feeding
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-77379433034
Y2  - 2024-11-22T02:01:49
ER  -