TY  - JOUR
AB  - Capsaicin has known pharmacological effects including the ability to reversibly open cellular tight junctions, among others. The aim of this study was to develop a strategy to enhance the paracellular transport of a substance with low permeability (FITC-dextran) across an epithelial cell monolayer via reversible opening of cellular tight junctions using a nanosystem comprised by capsaicin and of chitosan. We compared the biophysical properties of free capsaicin and capsaicin-loaded chitosan nanocapsules, including their cytotoxicity towards epithelial MDCK-C7 cells and their effect on the integrity of tight junctions, membrane permeability and cellular uptake. The cytotoxic response of MDCK-C7 cells to capsaicin at a concentration of 500 μM, which was evident for the free compound, is not observable following its encapsulation. The interaction between nanocapsules and the tight junctions of MDCK-C7 cells was investigated by impedance spectroscopy, digital holographic microscopy and structured illumination fluorescence microscopy. The nanocapsules modulated the interaction between capsaicin and tight junctions as shown by the different time profile of trans-epithelial electrical resistance and the enhanced permeability of monolayers incubated with FITC-dextran. Structured illumination fluorescence microscopy showed that the nanocapsules were internalized by MDCK-C7 cells. The capsaicin-loaded nanocapsules could be further developed as drug nanocarriers with enhanced epithelial permeability.
AU  - Kaiser, Mathias
AU  - Pereira, S.
AU  - Pohl, Luisa
AU  - Ketelhut, Steffi
AU  - Kemper, Björn
AU  - Gorzelanny, Christian
AU  - Galla, Hans-Joachim
AU  - Moerschbacher, Bruno
AU  - Goycoolea, Francisco Martin
DA  - 2015-05-13
DO  - 10.1038/srep10048
LA  - eng
N1  - Scientific Reports 5 (2015) 10048, 1-14
N1  - Finanziert durch den Open-Access-Publikationsfonds 2015/2016 der Westfälischen Wilhelms-Universität Münster (WWU Münster).
PY  - 2015-05-13
SN  - 2045-2322
TI  - Chitosan encapsulation modulates the effect of capsaicin on the tight junctions of MDCK cells
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-59229647377
Y2  - 2024-11-22T01:34:39
ER  -