TY  - JOUR
AB  - The presented work explores the regulatory influence of upstream open reading frames (uORFs) on gene expression in Trypanosoma congolense. More than 31,000 uORFs in total were identified and characterized here. We found evidence for the uORFs’ appearance in the transcriptome to be correlated with proteomic expression data, clearly indicating their repressive potential in T. congolense, which has to rely on post-transcriptional gene expression regulation due to its unique genomic organization. Our data show that uORF’s translation repressive potential does not only correlate with elemental sequence features such as length, position and quantity, but involves more subtle components, in particular the codon and amino acid profiles. This corresponds with the popular mechanistic model of a ribosome shedding initiation factors during the translation of a uORF, which can prevent reinitiation at the downstream start codon of the actual protein-coding sequence, due to the former extensive consumption of crucial translation components. We suggest that uORFs with uncommon codon and amino acid usage can slow down the translation elongation process in T. congolense, systematically deplete the limited factors, and restrict downstream reinitiation, setting up a bottleneck for subsequent translation of the protein-coding sequence. Additionally we conclude that uORFs dynamically influence the T. congolense life cycle. We found evidence that transition to epimastigote form could be supported by gain of uORFs due to alternative trans-splicing, which down-regulate housekeeping genes’ expression and render the trypanosome in a metabolically reduced state of endurance.
AU  - Fervers, Philipp Christopher
AU  - Fervers, Florian
AU  - Makałowski, Wojciech
AU  - Jąkalski, Marcin
DA  - 2018-08-09
DO  - 10.1371/journal.pone.0201461
LA  - eng
N1  - PLoS ONE 13 (2018) 8, e0201461, 1-26
N1  - Finanziert durch den Open-Access-Publikationsfonds 2018 der Deutschen Forschungsgemeinschaft (DFG) und der Westfälischen Wilhelms-Universität Münster (WWU Münster).
PY  - 2018-08-09
TI  - Life cycle adapted upstream open reading frames (uORFs) in 'Trypanosoma congolense': A post-transcriptional approach to accurate gene regulation
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-14199450922
Y2  - 2024-11-22T04:23:41
ER  -