TY  - JOUR
AB  - Chitin and chitosan oligomers have diverse biological activities with potentially valuable applications in fields like medicine, cosmetics, or agriculture. These properties may depend not only on the degrees of polymerization and acetylation, but also on a specific pattern of acetylation (PA) that cannot be controlled when the oligomers are produced by chemical hydrolysis. To determine the influence of the PA on the biological activities, defined chitosan oligomers in sufficient amounts are needed. Chitosan oligomers with specific PA can be produced by enzymatic deacetylation of chitin oligomers, but the diversity is limited by the low number of chitin deacetylases available. We have produced specific chitosan oligomers which are deacetylated at the first two units starting from the non-reducing end by the combined use of two different chitin deacetylases, namely NodB from Rhizobium sp. GRH2 that deacetylates the first unit and COD from Vibrio cholerae that deacetylates the second unit starting from the non-reducing end. Both chitin deacetylases accept the product of each other resulting in production of chitosan oligomers with a novel and defined PA. When extended to further chitin deacetylases, this approach has the potential to yield a large range of novel chitosan oligomers with a fully defined architecture.
AU  - Hamer, Stefanie
AU  - Hamer, Stefanie Nicole
AU  - Cord-Landwehr, Stefan
AU  - Landwehr, Stefan Cord-
AU  - Biarnés, Xevi
AU  - Planas, Antoni
AU  - Waegeman, Hendrik
AU  - Moerschbacher, Bruno
AU  - Kolkenbrock, Stephan
DA  - 2015-03-03
DO  - doi:10.1038/srep08716
LA  - eng
N1  - Scientific Reports 5 (2015) 8716, 1-9
N1  - Finanziert durch den Open-Access-Publikationsfonds 2014/2015 der Deutschen Forschungsgemeinschaft (DFG) und der Westfälischen Wilhelms-Universität Münster (WWU Münster).
PY  - 2015-03-03
SN  - 2045-2322
TI  - Enzymatic production of defined chitosan oligomers with a specific pattern of acetylation using a combination of chitin oligosaccharide deacetylases
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-90319481007
Y2  - 2024-11-22T08:11:21
ER  -