TY  - JOUR
AB  - We have developed a drug delivery nanosystem based on chitosan and capsaicin. Both substances have a wide range of biological activities. We investigated the nanosystem’s influence on migration and morphology of Madin Darby canine kidney (MDCK-C7) epithelial cells in comparison to the capsaicin-free nanoformulation, free capsaicin, and control cells. For minimally-invasive quantification of cell migration, we applied label-free digital holographic microscopy (DHM) and single-cell tracking. Moreover, quantitative DHM phase images were used as novel stain-free assay to quantify the temporal course of global cellular morphology changes in confluent cell layers. Cytoskeleton alterations and tight junction protein redistributions were complementary analyzed by fluorescence microscopy. Calcium influx measurements were conducted to characterize the influence of the nanoformulations and capsaicin on ion channel activities. We found that both, capsaicin-loaded and unloaded chitosan nanocapsules, and also free capsaicin, have a significant impact on directed cell migration and cellular motility. Increase of velocity and directionality of cell migration correlates with changes in the cell layer surface roughness, tight junction integrity and cytoskeleton alterations. Calcium influx into cells occurred only after nanoformulation treatment but not upon addition of free capsaicin. Our results pave the way for further studies on the biological significance of these findings and potential biomedical applications, e.g. as drug and gene carriers.
AU  - Kaiser, Mathias
AU  - Pohl, Luisa
AU  - Ketelhut, Steffi
AU  - Kastl, Lena
AU  - Gorzelanny, Christian
AU  - Götte, Martin
AU  - Schnekenburger, Jürgen
AU  - Goycoolea, Francisco Martin
AU  - Kemper, Björn
DA  - 2017-11-06
DO  - 10.1371/journal.pone.0187497
LA  - eng
N1  - PLoS ONE 12 (2017) 11, e0187497, 1-20
N1  - Finanziert durch den Open-Access-Publikationsfonds 2017 der Westfälischen Wilhelms-Universität Münster (WWU Münster).
PY  - 2017-11-06
TI  - Nanoencapsulated capsaicin changes migration behavior and morphology of madin darby canine kidney cell monolayers
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-66129494225
Y2  - 2024-11-21T16:52:29
ER  -