TY - JOUR AB - Detachment of cells from the primary tumour precedes metastatic progression by facilitating cell release into the tissue. Solid tumours exhibit altered pH homeostasis with extracellular acidification. In human melanoma, the Na+/H+ exchanger NHE1 is an important modifier of the tumour nanoenvironment. Here we tested the modulation of cell-cell-adhesion by extracellular pH and NHE1. MV3 tumour spheroids embedded in a collagen matrix unravelled the efficacy of cell-cell contact loosening and 3D emigration into an environment mimicking physiological confinement. Adhesive interaction strength between individual MV3 cells was quantified using atomic force microscopy and validated by multicellular aggregation assays. Extracellular acidification from pHe7.4 to 6.4 decreases cell migration and invasion but increases single cell detachment from the spheroids. Acidification and NHE1 overexpression both reduce cell-cell adhesion strength, indicated by reduced maximum pulling forces and adhesion energies. Multicellular aggregation and spheroid formation are strongly impaired under acidification or NHE1 overexpression. We show a clear dependence of melanoma cell-cell adhesion on pHe and NHE1 as a modulator. These effects are opposite to cell-matrix interactions that are strengthened by protons extruded via NHE1. We conclude that these opposite effects of NHE1 act synergistically during the metastatic cascade. AU - Hofschröer, Verena AU - Koch, Kevin Alexander AU - Ludwig, Florian Timo AU - Friedl, Peter AU - Oberleithner, Hans AU - Stock, Christian AU - Schwab, Jan Tilman DA - 2017-02-13 DO - 10.1038/srep42369 LA - eng N1 - Scientific Reports 7 (2017) 42369, 1-13 N1 - Finanziert durch den Open-Access-Publikationsfonds 2017 der Westfälischen Wilhelms-Universität Münster (WWU Münster). PY - 2017-02-13 TI - Extracellular protonation modulates cell-cell interaction mechanics and tissue invasion in human melanoma cells UR - https://nbn-resolving.org/urn:nbn:de:hbz:6-59129524259 Y2 - 2024-11-22T03:31:01 ER -