TY  - JOUR
AB  - Endothelial barriers have a central role in inflammation as they allow or deny the passage of leukocytes from the vasculature into the tissue. To bind leukocytes, endothelial cells form adhesive clusters containing tetraspanins and ICAM-1, so-called endothelial adhesive platforms (EAPs). Upon leukocyte binding, EAPs evolve into docking structures that emanate from the endothelial surface while engulfing the leukocyte. Here, we show that TNF-α is sufficient to induce apical protrusions in the absence of leukocytes. Using advanced quantitation of atomic force microscopy (AFM) recordings, we found these structures to protrude by 160 ± 80 nm above endothelial surface level. Confocal immunofluorescence microscopy proved them positive for ICAM-1, JAM-A, tetraspanin CD9 and f-actin. Microvilli formation was inhibited in the absence of CD9. Our findings indicate that stimulation with TNF-α induces nanoscale changes in endothelial surface architecture and that—via a tetraspanin CD9 depending mechanism—the EAPs rise above the surface to facilitate leukocyte capture.
AU  - Franz, Jonas
AU  - Brinkmann, Benjamin Franz
AU  - Brinkmann, Benjamin F.
AU  - König, Michael
AU  - Hüve, Jana
AU  - Stock, Christian
AU  - Ebnet, Klaus
AU  - Riethmüller, Christoph
DA  - 2016-01-05
DO  - 10.1371/journal.pone.0146598
LA  - eng
N1  - PLoS ONE 11 (2016) 1, e0146598, 1-20
N1  - Finanziert durch den Open-Access-Publikationsfonds 2015/2016 der Westfälischen Wilhelms-Universität Münster (WWU Münster).
PY  - 2016-01-05
SN  - 1932-6203
TI  - Nanoscale Imaging Reveals a Tetraspanin-CD9 Coordinated Elevation of Endothelial ICAM-1 Clusters
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-76229549436
Y2  - 2024-11-21T16:41:40
ER  -