TY  - JOUR
AB  - In the associated main paper (JPBA 2019), substance P was shown to be a valuable neuropeptide reporter substance to monitor the protease activity of serum. The assay was developed based on the predecessor assay using bradykinin (JPBA 2017). Both neuropeptides are of interest in inflammation and pain research and were thus explored for use with capillary blood and blood cards. Here, we present the protocols and set them in perspective to above neuropeptide assays for serum. Neuropeptide reporter substance protease activity assay for use with fresh and dried blood. Dabsylated Substance P and bradykinin are substrates of angiotensin-converting enzyme and other proteases. Neuropeptides of interest in inflammation and pain.
AU  - Schreiber, Ulrich
AU  - Engl, Christian
AU  - Bayer, Malte
AU  - König, Simone
DA  - 2020-07-04
DO  - 10.1016/j.mex.2020.100985
KW  - Bradykinin
KW  - Substance P
KW  - Thin-layer chromatography
KW  - Dried blood spots
KW  - Protease activity
LA  - eng
N1  - MethodsX 7 (2020) 100985, 1-5
N1  - Finanziert durch den Open-Access-Publikationsfonds der Westfälischen Wilhelms-Universität Münster (WWU Münster).
PY  - 2020-07-04
TI  - Neuropeptide reporter assay for serum, capillary blood and blood cards
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-60179417254
Y2  - 2024-11-24T18:27:44
ER  -