TY  - JOUR
AB  - A pseudo-MRM method using high-resolution mass spectrometry is presented for the specific detection of truncated tissue factor (tTF), retargeted to tumor vasculature by GNGRAHA peptide (tTF-NGR), in serum. In order to increase sensitivity, serum proteins are pre-separated by 1D-gel electrophoresis and the gel band corresponding to recombinant human tTF-NGR is investigated. Calibration is performed using the gelseparated pure protein. The method relies on six unique tryptic peptides and is sensitive at the low fmol level.
AU  - König, Simone
AU  - Harrach, Saliha
AU  - Berdel, Wolfgang E.
AU  - Berdel, Wolfgang
AU  - Berdel, W. E.
AU  - Schwöppe, Christian
DA  - 2017-10
KW  - mass spectrometry
KW  - protein quantification
KW  - serum
KW  - tumor
KW  - LC/MS
LA  - eng
IS  - Mercator Journal of Biomolecular Analysis
M2  - 22
PY  - 2017-10
SP  - 22-32
T2  - Mercator Journal of Biomolecular Analysis
TI  - Pseudo-MRM method for the selective detection of truncated tissue factor
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-00269468510
Y2  - 2024-11-22T05:07:40
ER  -