TY  - JOUR
AB  - Background: Therapeutic plasma exchange (TPE)-based protocols immediately before cadaveric donor kidney transplantation have been extensively used in highly sensitized recipients. Plasma is generally preferred over human albumin as replacement fluid to avoid depletion of coagulation factors and perioperative bleeding. The aim of this study was to estimate bleeding risk after TPE replaced with albumin using rotational thromboelastography (ROTEM). Methodology: Ten patients without overt coagulation abnormalities underwent TPE. Standard laboratory coagulation tests (thromboplastin time, activated partial thromboplastin time (aPTT), international normalized ratio (INR), thrombin clotting time, fibrinogen levels and antithrombin activity) were compared with thrombelastometry analysis (EXTEM and INTEM tests) before and after TPE. Principal Findings: TPE significantly reduced fibrinogen levels (482 ± 182 vs. 223 ± 122 mg/dL), antithrombin activity (103 ± 11 vs. 54 ± 11 %), and prolonged aPTT (28 ± 3 vs. 45 ± 8 s), thromboplastin time (108 ± 11 vs. 68 ± 11 %), INR (0.95 ± 0.06 vs. 1.25 ± 0.16), and thrombin clotting time (18 ± 2 vs. 20 ± 3 s). INTEM and EXTEM analyses revealed significantly prolonged clot-formation time and reduced maximum clot firmness. Conclusions/Significance: TPE replaced with albumin induces significant changes in global hemostasis parameters thus potentially increasing bleeding risk. Therefore, pretransplant TPE should be considered carefully in indicated patients before kidney transplantation. The role of the ROTEM point-of-care test to estimate the risk of bleeding in renal transplantation needs to be evaluated in further studies.
AU  - Thölking, Gerold Bernhard
AU  - Mesters, Rolf M.
AU  - Dittrich, Ralf
AU  - Pavenstädt, Hermann-Joseph
AU  - Kümpers, Philipp
AU  - Kümpers, Philipp Franz Gerhard
AU  - Reuter, Stefan Johannes
DA  - 2015-06-29
DO  - 10.1371/journal.pone.0130402
LA  - eng
N1  - PLoS ONE 10 (2015) 6, e0130402, 1-12
N1  - Finanziert durch den Open-Access-Publikationsfonds 2015/2016 der Westfälischen Wilhelms-Universität Münster (WWU Münster).
PY  - 2015-06-29
SN  - 1932-6203
TI  - Assessment of Hemostasis after Plasma Exchange Using Rotational Thrombelastometry (ROTEM)
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-38279550343
Y2  - 2024-11-22T01:01:26
ER  -