TY - JOUR AB - A pseudo-MRM method using high-resolution mass spectrometry is presented for the specific detection of HLA-G against a background of homologous proteins. The method relies on three unique and two unspecific tryptic peptides. The latter are used to monitor the level of background proteins in comparison to the desired HLA-G. Instrument response is linear in the range from 30 to 200 fmol. AU - König, Simone AU - Spurny, Christian AU - Rössig, Claudia DA - 2017-10 KW - mass spectrometry KW - protein quantification KW - serum KW - immune checkpoint molecule KW - LC/MS LA - eng IS - Mercator Journal of Biomolecular Analysis M2 - 12 PY - 2017-10 SP - 12-21 T2 - Mercator Journal of Biomolecular Analysis TI - Pseudo-MRM method for the selective detection of human HLA-G UR - https://nbn-resolving.org/urn:nbn:de:hbz:6-00269469431 Y2 - 2024-11-22T04:14:17 ER -