TY  - JOUR
AB  - Background: With socio-economic changes, dentists and maxillofacial surgeons are more and more faced with medically compromised patients. Especially, the admission of antirheumatic drugs has increased remarkably. So dentists and maxillofacial surgeons should be aware of related adverse reactions that affect the craniofacial region. To identify possible cellular effects of disease modifying antirheumatic drugs (DMARDs) we investigated the influence of methotrexate (MTX) on human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were incubated with various concentrations of MTX, corresponding to serum concentrations found in rheumatoid arthritis (RA) patients. The effect of MTX on cell proliferation, differentiation as well as mitochondrial activity was measured by use of immunostaining, cell counting and 3-(4, 5-dimethylthiazol-2-yl)- 2, 5-diphenyltetrazolium bromide (MTT) assay. Results: All samples incubated with MTX (1-1000 nM) showed significantly decreased cell viability when compared to controls. Cells were less proliferating, but did not lose their ability to synthesize endothelial proteins. A slight dose dependency of inhibiting effects was demonstrated. The observed differences between control and sample groups were rising with longer duration. Conclusion: Because of the crucial role of endothelial cells and their precursor cells in wound healing, a negative influence of MTX on oral health has to be supposed, correlating to clinical observations of adverse reactions in the oral cavity, such as ulcerative or erosive lesions.
AU  - Annussek, Tobias
AU  - Szuwart, Thomas
AU  - Kleinheinz, Johannes
AU  - Koiky, Cathrin
AU  - Wermker, Kai
DA  - 2014-05-22
DO  - doi:10.1186/1746-160X-10-19
KW  - Oral health
KW  - Antirheumatic drugs
KW  - Methotrexate
KW  - Endothelial cells
KW  - In vitro
KW  - Wound healing
LA  - eng
N1  - Head & Face Medicine 10 (2014) 19, 1-7
N1  - Finanziert durch den Open-Access-Publikationsfonds 2014/2015 der Deutschen Forschungsgemeinschaft (DFG) und der Westfälischen Wilhelms-Universität Münster (WWU Münster).
PY  - 2014-05-22
TI  - In vitro inhibition of HUVECs by low dose methotrexate - Insights into oral adverse events
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-01349481116
Y2  - 2024-11-22T03:59:07
ER  -