TY  - JOUR
AB  - Members of the red pigment-concentrating hormone (RPCH)/adipokinetic hormone (AKH) peptide family are involved in many physiological, developmental and behavioral processes in insects, and pigment translocation in decapod crustaceans. In the branchiopod Daphnia pulex, a preprohormone for RPCH/AKH is encoded in the genome and the mature peptide, Dappu-RPCH (pQVNFSTSW-amide) is predicted from the preprohormone. Here, we have developed a method to measure the peptide in hemolymph of Daphnia. It uses reversed-phase liquid chromatography and high-resolution mass spectrometry of the singly- and the doubly-charged peptide ions, respectively, and their gas phase fragmentation for target identification. Pilot studies were conducted with synthetic Dappu-RPCH and the method was sensitive at the low fmol level without biological matrix. When hemolymph samples were however spiked with known amounts of the synthetic peptide, much less peptide was recovered and detected. Thus far, with our method of peptide purification, the endogenous peptide was not detectable in hemolymph of Daphnia where AKH titres of 1 fmol/µl hemolymph are expected.
AU  - König, Simone
AU  - Beesoo, Rima
AU  - Hoffschröer, Nadine
AU  - Zeis, Bettina
AU  - Marco, Heather G.
DA  - 2020
KW  - Adipokinetisches Hormon
KW  - Massenspektrometrie
KW  - Hämolymphe
KW  - Adipokinetic hormone
KW  - Daphnia
KW  - mass spectrometry
KW  - hemolymph
LA  - eng
IS  - Mercator Journal of Biomolecular Analysis
M2  - 1
PY  - 2020
SP  - 1-6
T2  - Mercator Journal of Biomolecular Analysis
TI  - Target analysis for the adipokinetic hormone of 'Daphnia'
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-10139552201
Y2  - 2024-11-23T11:54:14
ER  -