TY  - JOUR
AB  - BACKGROUND: The production and employment of cellulases still represents an economic bottleneck in the conversion of lignocellulosic biomass to biofuels and other biocommodities. This process could be simplified by displaying the necessary enzymes on a microbial cell surface. Such an approach, however, requires an appropriate host organism which on the one hand can withstand the rough environment coming along with lignocellulose hydrolysis, and on the other hand does not consume the generated glucose so that it remains available for subsequent fermentation steps. RESULTS: The robust soil bacterium Pseudomonas putida showed a strongly reduced uptake of glucose above a temperature of 50 °C, while remaining structurally intact hence recyclable, which makes it suitable for cellulose hydrolysis at elevated temperatures. Consequently, three complementary, thermophilic cellulases from Ruminiclostridium thermocellum were displayed on the surface of the bacterium. All three enzymes retained their activity on the cell surface. A mixture of three strains displaying each one of these enzymes was able to synergistically hydrolyze filter paper at 55 °C, producing 20 μg glucose per mL cell suspension in 24 h. CONCLUSION: We could establish Pseudomonas putida as host for the surface display of cellulases, and provided proof-of-concept for a fast and simple cellulose breakdown process at elevated temperatures. This study opens up new perspectives for the application of P. putida in the production of biofuels and other biotechnological products.
AU  - Tozakidis, Iasson
AU  - Tozakidis, Iasson E. P.
AU  - Tozakidis, Iasson Elias Philippos
AU  - Brossette, Tatjana
AU  - Lenz, Florian
AU  - Maas, Ruth Maria
AU  - Jose, Joachim
DA  - 2016-06-10
DO  - 10.1186/s12934-016-0505-8
KW  - Whole cell biocatalysis
KW  - Cellulose breakdown
KW  - Glucose production
KW  - Pseudomonas putida
KW  - Cellulases
KW  - Surface display
KW  - Autotransporter
KW  - Maximized autotransporter mediated expression (MATE)
LA  - eng
N1  - Microbial Cell Factories 15 (2016) 103, 1-12
N1  - Finanziert durch den Open-Access-Publikationsfonds 2015/2016 der Westfälischen Wilhelms-Universität Münster (WWU Münster).
PY  - 2016-06-10
SN  - 1475-2859
TI  - Proof of concept for the simplified breakdown of cellulose by combining Pseudomonas putida strains with surface displayed thermophilic endocellulase, exocellulase and β-glucosidase
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-44279658582
Y2  - 2024-11-22T11:17:36
ER  -