TY  - JOUR
AB  - The autotrophic 3-hydroxypropionate/4-hydroxybutyrate (HP/HB) cycle functions in thermoacidophilic, (micro)aerobic, hydrogen-oxidizing 'Crenarchaeota' of the order 'Sulfolobales' as well as in mesophilic, aerobic, ammonia-oxidizing 'Thaumarchaeota'. Notably, the HP/HB cycle evolved independently in these two archaeal lineages, and crenarchaeal and thaumarchaeal versions differ regarding their enzyme properties and phylogeny. These differences result in altered energetic efficiencies between the variants. Compared to the crenarchaeal HP/HB cycle, the thaumarchaeal variant saves two ATP equivalents per turn, rendering it the most energy-efficient aerobic pathway for carbon fixation. Characteristically, the HP/HB cycle includes two enoyl coenzyme A (CoA) hydratase reactions: the 3-hydroxypropionyl-CoA dehydratase reaction and the crotonyl-CoA hydratase reaction. In this study, we show that both reactions are catalyzed in the aforementioned archaeal groups by a promiscuous 3-hydroxypropionyl-CoA dehydratase/crotonyl-CoA hydratase (Msed_2001 in crenarchaeon 'Metallosphaera sedula' and Nmar_1308 in thaumarchaeon 'Nitrosopumilus maritimus'). Although these two enzymes are homologous, they are closely related to bacterial enoyl-CoA hydratases and were retrieved independently from the same enzyme pool by the ancestors of 'Crenarchaeota' and 'Thaumarchaeota', despite the existence of multiple alternatives. This striking similarity in the emergence of enzymes involved in inorganic carbon fixation from two independently evolved pathways highlights that convergent evolution of autotrophy could be much more widespread than anticipated.
AU  - Liu, Li
AU  - Brown, Philip C.
AU  - Könneke, Martin
AU  - Huber, Harald
AU  - König, Simone
AU  - Berg, Ivan A.
DA  - 2021-01-20
DO  - 10.17879/83009533124
KW  - 3-hydroxypropionate/4-hydroxybutyrate cycle
KW  - 3-hydroxypropionyl-CoA dehydratase
KW  - Metallosphaera sedula
KW  - Nitrosopumilus maritimus
KW  - autotrophs
KW  - crotonyl-CoA hydratase
LA  - eng
N1  - mSphere 6 (2021) 1, e01079-20, 1-13
N1  - Finanziert durch den Open-Access-Publikationsfonds der Westfälischen Wilhelms-Universität Münster (WWU Münster).
N1  - Förderer: Deutsche Forschungsgemeinschaft / Projektnummer: BE 4822/5-1 und KO 3651/6-1
N1  - Funding organisation: Deutsche Forschungsgemeinschaft / Project number: BE 4822/5-1 und KO 3651/6-1
PY  - 2021-01-20
TI  - Convergent Evolution of a Promiscuous 3-Hydroxypropionyl-CoA Dehydratase/Crotonyl-CoA Hydratase in Crenarchaeota and Thaumarchaeota
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-82099524164
Y2  - 2024-11-22T01:30:04
ER  -