TY  - JOUR
AB  - During the past decade, detailed studies using well-defined ‘second generation’ chitosans have amply proved that both their material properties and their biological activities are dependent on their molecular structure, in particular on their degree of polymerisation (DP) and their fraction of acetylation (FA). Recent evidence suggests that the pattern of acetylation (PA), i.e., the sequence of acetylated and non-acetylated residues along the linear polymer, is equally important, but chitosan polymers with defined, non-random PA are not yet available. One way in which the PA will influence the bioactivities of chitosan polymers is their enzymatic degradation by sequence-dependent chitosan hydrolases present in the target tissues. The PA of the polymer substrates in conjunction with the subsite preferences of the hydrolases determine the type of oligomeric products and the kinetics of their production and further degradation. Thus, the bioactivities of chitosan polymers will at least in part be carried by the chitosan oligomers produced from them, possibly through their interaction with pattern recognition receptors in target cells. In contrast to polymers, partially acetylated chitosan oligosaccharides (paCOS) can be fully characterised concerning their DP, FA, and PA, and chitin deacetylases (CDAs) with different and known regio-selectivities are currently emerging as efficient tools to produce fully defined paCOS in quantities sufficient to probe their bioactivities. In this review, we describe the current state of the art on how CDAs can be used in forward and reverse mode to produce all of the possible paCOS dimers, trimers, and tetramers, most of the pentamers and many of the hexamers. In addition, we describe the biotechnological production of the required fully acetylated and fully deacetylated oligomer substrates, as well as the purification and characterisation of the paCOS products.
AU  - Bonin, Martin
AU  - Sreekumar, Sruthi
AU  - Cord-Landwehr, Stefan
AU  - Landwehr, Stefan Cord-
AU  - Moerschbacher, Bruno
DA  - 2020-10-22
DO  - 10.17879/84019615005
KW  - paCOS
KW  - chito-oligosaccharide
KW  - CDA
KW  - chitin deacetylase
KW  - pattern of acetylation
KW  - chitosan
KW  - chitin
KW  - chitinase
KW  - chitosanase
KW  - regio-selective
LA  - eng
N1  - International Journal of Molecular Sciences 21 (2020) 21, 7835, 1-22
N1  - Finanziert durch den Open-Access-Publikationsfonds der Westfälischen Wilhelms-Universität Münster (WWU Münster).
PY  - 2020-10-22
TI  - Preparation of Defined Chitosan Oligosaccharides Using Chitin Deacetylases
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-14019627539
Y2  - 2024-11-22T04:25:02
ER  -