TY  - JOUR
AB  - Endometriosis is a painful gynecological condition characterized by ectopic growth of endometrial cells. Little is known about its pathogenesis, which is partially due to a lack of suitable experimental models. Here, we use endometrial stromal (St-T1b), primary endometriotic stromal, epithelial endometriotic (12Z) and co-culture (1:1 St-T1b:12Z) spheroids to mimic the architecture of endometrium, and either collagen I or Matrigel to model ectopic locations. Stromal spheroids, but not single cells, assumed coordinated directional migration followed by matrix remodeling of collagen I on day 5 or 7, resembling ectopic lesions. While generally a higher area fold increase of spheroids occurred on collagen I compared to Matrigel, directional migration was not observed in co-culture or in 12Z cells. The fold increase in area on collagen I was significantly reduced by MMP inhibition in stromal but not 12Z cells. Inhibiting ROCK signalling responsible for actomyosin contraction increased the fold increase of area and metabolic activity compared to untreated controls on Matrigel. The number of protrusions emanating from 12Z spheroids on Matrigel was decreased by microRNA miR-200b and increased by miR-145. This study demonstrates that spheroid assay is a promising pre-clinical tool that can be used to evaluate small molecule drugs and microRNA-based therapeutics for endometriosis.
AU  - Stejskalová, Anna
AU  - Fincke, Victoria
AU  - Nowak, Melissa K.
AU  - Nowak, Melissa Kim
AU  - Nowak, Melissa
AU  - Schmidt, Yvonne
AU  - Borrmann, Katrin
AU  - Wahlde, Marie‑Kristin von
AU  - Schäfer, Sebastian Daniel
AU  - Kiesel, Ludwig
AU  - Greve, Burkhard
AU  - Götte, Martin
DA  - 2021-02-18
DO  - 10.17879/22049533316
KW  - Experimental models of disease
KW  - Preclinical research
KW  - Reproductive disorders
LA  - eng
N1  - Scientific Reports 11 (2021), 4115, 1-15
N1  - Finanziert über die DEAL-Vereinbarung mit Wiley 2019-2022.
PY  - 2021-02-18
TI  - Collagen I triggers directional migration, invasion and matrix remodeling of stroma cells in a 3D spheroid model of endometriosis
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-22049529306
Y2  - 2024-11-22T03:52:27
ER  -